Orto Botanico "Pietro Castelli" dell'Università di Messina

The Orto Botanico "Pietro Castelli" dell'Università di Messina (8000 m²), also known as the Orto botanico di Messina, is a botanical garden operated by the University of Messina and located at Piazza 20 Settembre, Messina, Sicily, Italy. The university's first botanical garden was established in 1638 by Pietro Castelli, but destroyed by the Spanish along with the rest of the university in 1678.

Although in 1889 the garden was reestablished on the banks of the river Portalegni, this second version was ruined by the earthquake of 1908, and most of its site then devoted to building construction.